Design Primer by Variation ID:

This tool is intended for researchers to pick PCR-primers to validate SNPs/INDELs or develop molecular markers, using Primer3 as a backend engine. Please input one SNP or INDEL ID (e.g., vg0701959554) and select upstream and downstream regions (default is 100 bp). All the parameters can be changed if necessary.


Upstream:
Downstream:

Parameter Selection:

Optimal primer size:
Max primer size:
Min primer size:
Optimal primer TM:
Max primer TM:
Min primer TM:
Optimal GC percent:
MAX GC percent:
MIN GC percent:
Max #N's accepted:
Max Poly-X:
Max Internal Poly-X:
Max Self Complementarity:
Max 3' Self Complementarity:
Max Pair Complementarity:
Max 3' Pair Complementarity:
Product Size Ranges: